Note the change in statistics in the Graph Window as well as the workspace. Expand the Active Gate drop-down menu and click the “-” to shrink or the “+” to expand. To use it, first draw a gate with anything but the rectangle tool. All polygon (not quadrant) except the rectangle tool can be expanded or contracted using this feature. Expanding or Contracting the Gate SizeĪ new feature in FlowJo v10 allows you to symmetrically adjust a gate’s size via the Active Gate drop-down menu. When multiple gates exist in one graph window, you can navigate between the gates by simply clicking on the gates you wish to select. For finer movements, select a gate and use the arrow keys on the keyboard to move the gate one pixel in the direction of the arrow key. If you want to move the entire gate, mouse within the selected gate (the cursor will change to a four-sided arrow), then click-and-drag it to the new location. FlowJo will also adjust the statistics column in the workspace and any other statistics that are dependent on the adjusted gate. Note the updated statistic within the red boxes in both graphs. To move a vertex, select a gate, click on the vertex handle (black square, highlighted by a red circle below) and hold the mouse button down while dragging the vertex to a new location (shown below).īefore moving the vertex After moving the vertex FlowJo’s gating tools allow you to increase and decrease the size of a gate, change a vertex (or many), expand or contract along a contour line, move the entire position of the gate or delete a gate entirely. Need to tighten that gate to remove doublets or debris? Was that lymphocyte gate too generous? FlowJo allows you to adjust gates at any point during your analysis with immediate updates to statistics, tables and layouts.Īnalyzing flow cytometry data often requires adjustment to previously defined gates in order to accomodate or remove certain groups of events.
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